Back to Multiple platform build/check report for BioC 3.17:   simplified   long
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This page was generated on 2023-06-05 11:05:09 -0400 (Mon, 05 Jun 2023).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo1Linux (Ubuntu 22.04.2 LTS)x86_644.3.0 RC (2023-04-13 r84269) -- "Already Tomorrow" 4623
palomino3Windows Server 2022 Datacenterx644.3.0 RC (2023-04-13 r84269 ucrt) -- "Already Tomorrow" 4374
merida1macOS 12.6.4 Montereyx86_644.3.0 RC (2023-04-13 r84257) -- "Already Tomorrow" 4332
Click on any hostname to see more info about the system (e.g. compilers)      (*) as reported by 'uname -p', except on Windows and Mac OS X

CHECK results for SpliceWiz on palomino3


To the developers/maintainers of the SpliceWiz package:
- Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/SpliceWiz.git to reflect on this report. See Troubleshooting Build Report for more information.

- Use the following Renviron settings to reproduce errors and warnings.

Note: If "R CMD check" recently failed on the Linux builder over a missing dependency, add the missing dependency to "Suggests" in your DESCRIPTION file. See the Renviron.bioc for details.

raw results

Package 1992/2229HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
SpliceWiz 1.2.1  (landing page)
Alex Chit Hei Wong
Snapshot Date: 2023-06-04 14:00:14 -0400 (Sun, 04 Jun 2023)
git_url: https://git.bioconductor.org/packages/SpliceWiz
git_branch: RELEASE_3_17
git_last_commit: d3a2512
git_last_commit_date: 2023-05-20 02:32:04 -0400 (Sat, 20 May 2023)
nebbiolo1Linux (Ubuntu 22.04.2 LTS) / x86_64  OK    OK    OK  UNNEEDED, same version is already published
palomino3Windows Server 2022 Datacenter / x64  OK    OK    OK    OK  UNNEEDED, same version is already published
merida1macOS 12.6.4 Monterey / x86_64  OK    OK    TIMEOUT    NA  

Summary

Package: SpliceWiz
Version: 1.2.1
Command: F:\biocbuild\bbs-3.17-bioc\R\bin\R.exe CMD check --no-multiarch --install=check:SpliceWiz.install-out.txt --library=F:\biocbuild\bbs-3.17-bioc\R\library --no-vignettes --timings SpliceWiz_1.2.1.tar.gz
StartedAt: 2023-06-05 07:13:31 -0400 (Mon, 05 Jun 2023)
EndedAt: 2023-06-05 07:26:31 -0400 (Mon, 05 Jun 2023)
EllapsedTime: 780.5 seconds
RetCode: 0
Status:   OK  
CheckDir: SpliceWiz.Rcheck
Warnings: 0

Command output

##############################################################################
##############################################################################
###
### Running command:
###
###   F:\biocbuild\bbs-3.17-bioc\R\bin\R.exe CMD check --no-multiarch --install=check:SpliceWiz.install-out.txt --library=F:\biocbuild\bbs-3.17-bioc\R\library --no-vignettes --timings SpliceWiz_1.2.1.tar.gz
###
##############################################################################
##############################################################################


* using log directory 'F:/biocbuild/bbs-3.17-bioc/meat/SpliceWiz.Rcheck'
* using R version 4.3.0 RC (2023-04-13 r84269 ucrt)
* using platform: x86_64-w64-mingw32 (64-bit)
* R was compiled by
    gcc.exe (GCC) 12.2.0
    GNU Fortran (GCC) 12.2.0
* running under: Windows Server 2022 x64 (build 20348)
* using session charset: UTF-8
* using option '--no-vignettes'
* checking for file 'SpliceWiz/DESCRIPTION' ... OK
* this is package 'SpliceWiz' version '1.2.1'
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking whether package 'SpliceWiz' can be installed ... OK
* used C++ compiler: 'G__~1.EXE (GCC) 12.2.0'
* checking C++ specification ... NOTE
  Specified C++11: please drop specification unless essential
* checking installed package size ... OK
* checking package directory ... OK
* checking 'build' directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking startup messages can be suppressed ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in shell scripts ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking line endings in Makefiles ... OK
* checking compilation flags in Makevars ... OK
* checking for GNU extensions in Makefiles ... OK
* checking for portable use of $(BLAS_LIBS) and $(LAPACK_LIBS) ... OK
* checking use of PKG_*FLAGS in Makefiles ... OK
* checking compiled code ... NOTE
Note: information on .o files for x64 is not available
File 'F:/biocbuild/bbs-3.17-bioc/R/library/SpliceWiz/libs/x64/SpliceWiz.dll':
  Found '_assert', possibly from 'assert' (C)
  Found 'abort', possibly from 'abort' (C), 'runtime' (Fortran)
  Found 'exit', possibly from 'exit' (C), 'stop' (Fortran)

Compiled code should not call entry points which might terminate R nor
write to stdout/stderr instead of to the console, nor use Fortran I/O
nor system RNGs nor [v]sprintf. The detected symbols are linked into
the code but might come from libraries and not actually be called.

See 'Writing portable packages' in the 'Writing R Extensions' manual.
* checking files in 'vignettes' ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
                         user system elapsed
Build-Reference-methods 66.86   2.01   77.67
NxtSE-class             52.98   1.00   54.36
ASE-methods             27.36   0.21   27.59
covPlotObject-class     18.32   0.46   18.78
plotCoverage            12.28   0.20   12.50
Run_SpliceWiz_Filters    7.21   0.05    7.25
* checking for unstated dependencies in 'tests' ... OK
* checking tests ...
  Running 'testthat.R'
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in 'inst/doc' ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 2 NOTEs
See
  'F:/biocbuild/bbs-3.17-bioc/meat/SpliceWiz.Rcheck/00check.log'
for details.



Installation output

SpliceWiz.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   F:\biocbuild\bbs-3.17-bioc\R\bin\R.exe CMD INSTALL SpliceWiz
###
##############################################################################
##############################################################################


* installing to library 'F:/biocbuild/bbs-3.17-bioc/R/library'
* installing *source* package 'SpliceWiz' ...
** using staged installation
** libs
using C++ compiler: 'G__~1.EXE (GCC) 12.2.0'
using C++11
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c BAM2blocks.cpp -o BAM2blocks.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c FastaReader.cpp -o FastaReader.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c FragmentBlocks.cpp -o FragmentBlocks.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c GZTools.cpp -o GZTools.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c RcppExports.cpp -o RcppExports.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c ReadBlockProcessor.cpp -o ReadBlockProcessor.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c ReadBlockProcessor_CoverageBlocks.cpp -o ReadBlockProcessor_CoverageBlocks.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c ReadBlockProcessor_FragmentsMap.cpp -o ReadBlockProcessor_FragmentsMap.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c ReadBlockProcessor_TandemJunctions.cpp -o ReadBlockProcessor_TandemJunctions.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c covTools.cpp -o covTools.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c main.cpp -o main.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c swEngine.cpp -o swEngine.o
g++  -std=gnu++11 -I"F:/biocbuild/bbs-3.17-bioc/R/include" -DNDEBUG  -I'F:/biocbuild/bbs-3.17-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.17-bioc/R/library/RcppProgress/include'   -I"C:/rtools43/x86_64-w64-mingw32.static.posix/include"  -fopenmp -DSPLICEWIZ   -O2 -Wall  -mfpmath=sse -msse2 -mstackrealign  -c synthReadGenerator.cpp -o synthReadGenerator.o
g++ -shared -s -static-libgcc -o SpliceWiz.dll tmp.def BAM2blocks.o FastaReader.o FragmentBlocks.o GZTools.o RcppExports.o ReadBlockProcessor.o ReadBlockProcessor_CoverageBlocks.o ReadBlockProcessor_FragmentsMap.o ReadBlockProcessor_TandemJunctions.o covTools.o main.o swEngine.o synthReadGenerator.o -fopenmp -DSPLICEWIZ -LF:/biocbuild/bbs-3.17-bioc/R/library/zlibbioc/libs/x64 -lzlib1bioc -LC:/rtools43/x86_64-w64-mingw32.static.posix/lib/x64 -LC:/rtools43/x86_64-w64-mingw32.static.posix/lib -LF:/biocbuild/bbs-3.17-bioc/R/bin/x64 -lR
installing to F:/biocbuild/bbs-3.17-bioc/R/library/00LOCK-SpliceWiz/00new/SpliceWiz/libs/x64
** R
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (SpliceWiz)

Tests output

SpliceWiz.Rcheck/tests/testthat.Rout


R version 4.3.0 RC (2023-04-13 r84269 ucrt) -- "Already Tomorrow"
Copyright (C) 2023 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64 (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(SpliceWiz)
Loading required package: NxtIRFdata
SpliceWiz package loaded with 2 threads
Use setSWthreads() to set the number of SpliceWiz threads
> 
> test_check("SpliceWiz")
Loading required package: Rsubread
Jun 05 7:23:13 AM Converting FASTA to local TwoBitFile...done
Jun 05 7:23:14 AM Connecting to genome TwoBitFile...done
Jun 05 7:23:14 AM Making local copy of GTF file...done
Jun 05 7:23:15 AM Extracting temp genome FASTA from TwoBit file
Jun 05 7:23:16 AM Generating synthetic reads, saving to F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\refWithMapExcl/Mappability/Reads.fa
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
10145 synthetic reads generated
Jun 05 7:23:16 AM Cleaning temp genome / gene annotation files

        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
       Rsubread 2.14.2

//================================= setting ==================================\\
||                                                                            ||
||                Index name : reference_index                                ||
||               Index space : base space                                     ||
||               Index split : no-split                                       ||
||          Repeat threshold : 100 repeats                                    ||
||              Gapped index : no                                             ||
||                                                                            ||
||       Free / total memory : 41.5GB / 64.0GB                                ||
||                                                                            ||
||               Input files : 1 file in total                                ||
||                             o genome.fa                                    ||
||                                                                            ||
\\============================================================================//

//================================= Running ==================================\\
||                                                                            ||
|| Check the integrity of provided reference sequences ...                    ||
|| No format issues were found                                                ||
|| Scan uninformative subreads in reference sequences ...                     ||
|| Estimate the index size...                                                 ||
||    8%,   0 mins elapsed, rate=68.6k bps/s                                  ||
||   16%,   0 mins elapsed, rate=133.6k bps/s                                 ||
||   24%,   0 mins elapsed, rate=195.6k bps/s                                 ||
||   33%,   0 mins elapsed, rate=254.8k bps/s                                 ||
||   41%,   0 mins elapsed, rate=311.7k bps/s                                 ||
||   49%,   0 mins elapsed, rate=366.6k bps/s                                 ||
||   58%,   0 mins elapsed, rate=418.6k bps/s                                 ||
||   66%,   0 mins elapsed, rate=469.0k bps/s                                 ||
||   74%,   0 mins elapsed, rate=517.7k bps/s                                 ||
||   83%,   0 mins elapsed, rate=564.6k bps/s                                 ||
||   91%,   0 mins elapsed, rate=609.6k bps/s                                 ||
|| 3.0 GB of memory is needed for index building.                             ||
|| Build the index...                                                         ||
||    8%,   0 mins elapsed, rate=6.6k bps/s                                   ||
||   16%,   0 mins elapsed, rate=13.2k bps/s                                  ||
||   24%,   0 mins elapsed, rate=19.8k bps/s                                  ||
||   33%,   0 mins elapsed, rate=26.3k bps/s                                  ||
||   41%,   0 mins elapsed, rate=32.8k bps/s                                  ||
||   49%,   0 mins elapsed, rate=39.2k bps/s                                  ||
||   58%,   0 mins elapsed, rate=45.6k bps/s                                  ||
||   66%,   0 mins elapsed, rate=52.0k bps/s                                  ||
||   74%,   0 mins elapsed, rate=58.3k bps/s                                  ||
||   83%,   0 mins elapsed, rate=64.6k bps/s                                  ||
||   91%,   0 mins elapsed, rate=70.8k bps/s                                  ||
|| Save current index block...                                                ||
||  [ 0.0% finished ]                                                         ||
||  [ 10.0% finished ]                                                        ||
||  [ 20.0% finished ]                                                        ||
||  [ 30.0% finished ]                                                        ||
||  [ 40.0% finished ]                                                        ||
||  [ 50.0% finished ]                                                        ||
||  [ 60.0% finished ]                                                        ||
||  [ 70.0% finished ]                                                        ||
||  [ 80.0% finished ]                                                        ||
||  [ 90.0% finished ]                                                        ||
||  [ 100.0% finished ]                                                       ||
||                                                                            ||
||                      Total running time: 0.2 minutes.                      ||
||Index F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\refWithMapExcl\refe ... ||
||                                                                            ||
\\============================================================================//


        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
       Rsubread 2.14.2

//================================= setting ==================================\\
||                                                                            ||
|| Function      : Read alignment + Junction detection (RNA-Seq)              ||
|| Input file    : Reads.fa                                                   ||
|| Output file   : AlignedReads.bam (BAM)                                     ||
|| Index name    : reference_index                                            ||
||                                                                            ||
||                    ------------------------------------                    ||
||                                                                            ||
||                               Threads : 1                                  ||
||                          Phred offset : 33                                 ||
||                             Min votes : 1 / 14                             ||
||                        Max mismatches : 3                                  ||
||                      Max indel length : 5                                  ||
||            Report multi-mapping reads : yes                                ||
|| Max alignments per multi-mapping read : 1                                  ||
||                           Annotations : transcripts.gtf (GTF)              ||
||                                                                            ||
\\============================================================================//

//================ Running (05-Jun-2023 07:23:28, pid=59692) =================\\
||                                                                            ||
|| Check the input reads.                                                     ||
|| The input file contains base space reads.                                  ||
|| Initialise the memory objects.                                             ||
|| Estimate the mean read length.                                             ||
|| Create the output BAM file.                                                ||
|| Check the index.                                                           ||
|| Init the voting space.                                                     ||
|| Load the annotation file.                                                  ||
|| 538 annotation records were loaded.                                        ||
|| Global environment is initialised.                                         ||
|| Load the 1-th index block...                                               ||
|| The index block has been loaded.                                           ||
|| Start read mapping in chunk.                                               ||
||   65% completed, 0.4 mins elapsed, rate=36.4k reads per second             ||
||   83% completed, 0.4 mins elapsed, rate=0.4k reads per second              ||
||   99% completed, 0.4 mins elapsed, rate=0.5k reads per second              ||
||                                                                            ||
||                           Completed successfully.                          ||
||                                                                            ||
\\====================================    ====================================//

//================================   Summary =================================\\
||                                                                            ||
||                 Total reads : 10145                                        ||
||                      Mapped : 10145 (100.0%)                               ||
||             Uniquely mapped : 10145                                        ||
||               Multi-mapping : 0                                            ||
||                                                                            ||
||                    Unmapped : 0                                            ||
||                                                                            ||
||                   Junctions : 0                                            ||
||                      Indels : 1                                            ||
||                                                                            ||
||                Running time : 0.4 minutes                                  ||
||                                                                            ||
\\============================================================================//

Jun 05 7:23:50 AM Calculating Mappability Exclusion regions from: F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK/refWithMapExcl/Mappability/AlignedReads.bam
Calculating Mappability Exclusions: F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\refWithMapExcl\Mappability\AlignedReads.bam
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing Mappability Exclusions
**************************************************|
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Jun 05 7:23:54 AM Reference generated without non-polyA reference
Jun 05 7:23:54 AM Reference generated without Mappability reference
Jun 05 7:23:54 AM Reference generated without Blacklist exclusion
Jun 05 7:23:54 AM Converting FASTA to local TwoBitFile...done
Jun 05 7:23:55 AM Connecting to genome TwoBitFile...done
Jun 05 7:23:55 AM Making local copy of GTF file...done
Jun 05 7:23:56 AM Reading source GTF file...done
Jun 05 7:23:57 AM Processing gtf file...
...genes
...transcripts
...CDS
...exons
done
Jun 05 7:23:58 AM Gene ontology not prepared for this reference
Jun 05 7:24:00 AM Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Jun 05 7:24:04 AM Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Jun 05 7:24:12 AM Predicting NMD transcripts from genome sequence
...exonic transcripts
...retained introns

  |                                                                            
  |                                                                      |   0%
  |                                                                            
  |======================================================================| 100%
done
Jun 05 7:24:14 AM Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Jun 05 7:24:16 AM Splice Annotations Filtered
Jun 05 7:24:17 AM Translating Alternate Splice Peptides...done
Jun 05 7:24:17 AM Splice Annotations finished

Reference build finished
Jun 05 7:24:18 AM Running SpliceWiz processBAM
Reading reference file
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
Processing BAM file F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H003.bam
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing COV file
**************************************************|
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H003.bam processed (347 milliseconds)
Jun 05 7:24:19 AM Running SpliceWiz processBAM
Reading reference file
Allocating memory to 2 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
Processing BAM file F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H003.bam
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Compiling data from threads
Performing final sort of fragment maps
Writing COV file
**************************************************|
0%   10   20   30   40   50   60   70   80   90   100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H003.bam processed (289 milliseconds)
Jun 05 7:24:19 AM SpliceWiz reference already exists in given directory
Jun 05 7:24:19 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H003.bam processed (362 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H025.bam processed (315 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H026.bam processed (578 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H033.bam processed (637 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H043.bam processed (383 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H046.bam processed (466 milliseconds)
Jun 05 7:24:22 AM Validating Experiment; checking COV files...
Jun 05 7:24:22 AM Compiling Sample Stats
Jun 05 7:24:22 AM Compiling Junction List...merging...done
Jun 05 7:24:23 AM Compiling Junction Stats...merging...done
Jun 05 7:24:31 AM Compiling Intron Retention List...done
Jun 05 7:24:32 AM Compiling Tandem Junction List...merging...done
Jun 05 7:24:33 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...looking for novel exons
Jun 05 7:24:36 AM Assembling novel splicing reference:
...loading reference FASTA/GTF
...injecting novel transcripts to GTF
...processing GTF
...processing introns from GTF
...annotating alternative splicing events
done
Jun 05 7:24:49 AM Tidying up splice junctions and intron retentions (part 2)...
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done

Jun 05 7:24:57 AM Generating NxtSE assays
Jun 05 7:25:12 AM Building Final NxtSE Object
Jun 05 7:25:12 AM ...consolidating assays to H5 file
Jun 05 7:25:14 AM ...packaging reference
Jun 05 7:25:14 AM ...synthesising NxtSE
Jun 05 7:25:14 AM ...determining how overlapping introns should be removed
Jun 05 7:25:21 AM SpliceWiz (NxtSE) Collation Finished
Jun 05 7:25:21 AM Loading NxtSE object from file...
Jun 05 7:25:21 AM ...loading reference (NxtSE)
Jun 05 7:25:21 AM ...linking assays
Jun 05 7:25:21 AM ...linking COV files
Jun 05 7:25:21 AM ...loading rowData
Jun 05 7:25:21 AM ...removing overlapping introns...
Jun 05 7:25:21 AM NxtSE loaded
Jun 05 7:25:22 AM SpliceWiz reference already exists in given directory
Jun 05 7:25:22 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H003.bam processed (462 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H025.bam processed (378 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H026.bam processed (582 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H033.bam processed (592 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H043.bam processed (380 milliseconds)
F:\biocbuild\bbs-3.17-bioc\tmpdir\RtmpiGHigK\02H046.bam processed (448 milliseconds)
Jun 05 7:25:24 AM Validating Experiment; checking COV files...
Jun 05 7:25:24 AM Compiling Sample Stats
Jun 05 7:25:24 AM Compiling Junction List...merging...done
Jun 05 7:25:26 AM Compiling Junction Stats...merging...done
Jun 05 7:25:33 AM Compiling Intron Retention List...done
Jun 05 7:25:34 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...copying splicing reference
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done

Jun 05 7:25:45 AM Generating NxtSE assays
Jun 05 7:26:01 AM Building Final NxtSE Object
Jun 05 7:26:01 AM ...consolidating assays to H5 file
Jun 05 7:26:03 AM ...packaging reference
Jun 05 7:26:03 AM ...synthesising NxtSE
Jun 05 7:26:03 AM ...determining how overlapping introns should be removed
Jun 05 7:26:09 AM SpliceWiz (NxtSE) Collation Finished
Jun 05 7:26:09 AM Loading NxtSE object from file...
Jun 05 7:26:09 AM ...loading reference (NxtSE)
Jun 05 7:26:09 AM ...linking assays
Jun 05 7:26:09 AM ...linking COV files
Jun 05 7:26:09 AM ...loading rowData
Jun 05 7:26:09 AM ...removing overlapping introns...
Jun 05 7:26:09 AM NxtSE loaded
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 29 ]
> 
> # bump to trigger r cmd check
> 
> proc.time()
   user  system elapsed 
 181.59    6.70  191.53 

Example timings

SpliceWiz.Rcheck/SpliceWiz-Ex.timings

nameusersystemelapsed
ASE-GLM-edgeR2.640.102.73
ASE-methods27.36 0.2127.59
ASEFilter-class0.040.000.05
Build-Reference-methods66.86 2.0177.67
Coverage1.110.081.19
Gene-ontology-methods1.330.081.40
Graphics-User-Interface000
Mappability-methods2.610.053.36
NxtSE-class52.98 1.0054.36
Run_SpliceWiz_Filters7.210.057.25
STAR-methods000
View-Reference-methods0.150.000.15
collateData0.270.060.33
coord2GR0.030.000.03
covDataObject-class1.610.011.67
covPlotObject-class18.32 0.4618.78
covPlotly-class1.080.031.11
example-SpliceWiz-data0.010.000.01
findSamples0.020.000.02
isCOV0.010.000.01
makeSE0.540.140.67
make_plot_data0.030.020.05
plotCoverage12.28 0.2012.50
processBAM2.500.101.59
setSWthreads000
theme_white0.120.000.13