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This page was generated on 2023-03-24 11:06:38 -0400 (Fri, 24 Mar 2023).

HostnameOSArch (*)R versionInstalled pkgs
nebbiolo2Linux (Ubuntu 20.04.5 LTS)x86_644.2.3 (2023-03-15) -- "Shortstop Beagle" 4286
palomino4Windows Server 2022 Datacenterx644.2.3 (2023-03-15 ucrt) -- "Shortstop Beagle" 4276
lconwaymacOS 12.5.1 Montereyx86_644.2.3 (2023-03-15) -- "Shortstop Beagle" 4303
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CHECK results for GUIDEseq on lconway


To the developers/maintainers of the GUIDEseq package:
- Please allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/GUIDEseq.git to
reflect on this report. See How and When does the builder pull? When will my changes propagate? for more information.
- Make sure to use the following settings in order to reproduce any error or warning you see on this page.

raw results

Package 879/2183HostnameOS / ArchINSTALLBUILDCHECKBUILD BIN
GUIDEseq 1.28.0  (landing page)
Lihua Julie Zhu
Snapshot Date: 2023-03-22 14:00:04 -0400 (Wed, 22 Mar 2023)
git_url: https://git.bioconductor.org/packages/GUIDEseq
git_branch: RELEASE_3_16
git_last_commit: e29dcac
git_last_commit_date: 2022-11-01 11:12:23 -0400 (Tue, 01 Nov 2022)
nebbiolo2Linux (Ubuntu 20.04.5 LTS) / x86_64  OK    OK    OK  UNNEEDED, same version is already published
palomino4Windows Server 2022 Datacenter / x64  OK    OK    OK    OK  UNNEEDED, same version is already published
lconwaymacOS 12.5.1 Monterey / x86_64  OK    OK    OK    OK  UNNEEDED, same version is already published

Summary

Package: GUIDEseq
Version: 1.28.0
Command: /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:GUIDEseq.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings GUIDEseq_1.28.0.tar.gz
StartedAt: 2023-03-22 20:39:58 -0400 (Wed, 22 Mar 2023)
EndedAt: 2023-03-22 20:47:18 -0400 (Wed, 22 Mar 2023)
EllapsedTime: 440.1 seconds
RetCode: 0
Status:   OK  
CheckDir: GUIDEseq.Rcheck
Warnings: 0

Command output

##############################################################################
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###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD check --install=check:GUIDEseq.install-out.txt --library=/Library/Frameworks/R.framework/Resources/library --no-vignettes --timings GUIDEseq_1.28.0.tar.gz
###
##############################################################################
##############################################################################


* using log directory ‘/Users/biocbuild/bbs-3.16-bioc/meat/GUIDEseq.Rcheck’
* using R version 4.2.3 (2023-03-15)
* using platform: x86_64-apple-darwin17.0 (64-bit)
* using session charset: UTF-8
* using option ‘--no-vignettes’
* checking for file ‘GUIDEseq/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘GUIDEseq’ version ‘1.28.0’
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘GUIDEseq’ can be installed ... OK
* checking installed package size ... NOTE
  installed size is 12.4Mb
  sub-directories of 1Mb or more:
    extdata  12.0Mb
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking dependencies in R code ... NOTE
':::' call which should be '::': 'CRISPRseek:::translatePattern'
  See the note in ?`:::` about the use of this operator.
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... NOTE
.maskSubSeq: no visible global function definition for '.getMatchedInd'
.nucleotideSubstitutionMatrix: no visible binding for global variable
  'IUPAC_CODE_MAP'
.nucleotideSubstitutionMatrix: no visible binding for global variable
  'DNA_BASES'
GUIDEseqAnalysis: no visible binding for global variable 'offTarget'
GUIDEseqAnalysis: no visible binding for global variable 'peak_score'
GUIDEseqAnalysis: no visible binding for global variable
  'predicted_cleavage_score'
GUIDEseqAnalysis: no visible binding for global variable 'gRNA.name'
GUIDEseqAnalysis: no visible binding for global variable 'gRNAPlusPAM'
GUIDEseqAnalysis: no visible binding for global variable
  'offTarget_sequence'
GUIDEseqAnalysis: no visible binding for global variable
  'guideAlignment2OffTarget'
GUIDEseqAnalysis: no visible binding for global variable
  'offTargetStrand'
GUIDEseqAnalysis: no visible binding for global variable
  'mismatch.distance2PAM'
GUIDEseqAnalysis: no visible binding for global variable
  'n.guide.mismatch'
GUIDEseqAnalysis: no visible binding for global variable
  'offTarget_Start'
GUIDEseqAnalysis: no visible binding for global variable
  'offTarget_End'
GUIDEseqAnalysis: no visible binding for global variable 'chromosome'
GUIDEseqAnalysis: no visible binding for global variable
  'gRNA.insertion'
GUIDEseqAnalysis: no visible binding for global variable
  'gRNA.deletion'
GUIDEseqAnalysis: no visible binding for global variable
  'pos.insertion'
GUIDEseqAnalysis: no visible binding for global variable 'pos.deletion'
GUIDEseqAnalysis: no visible binding for global variable 'n.insertion'
GUIDEseqAnalysis: no visible binding for global variable 'n.deletion'
GUIDEseqAnalysis: no visible binding for global variable 'n.RNA.bulge'
GUIDEseqAnalysis: no visible binding for global variable 'n.DNA.bulge'
GUIDEseqAnalysis: no visible binding for global variable 'feature'
GUIDEseqAnalysis: no visible binding for global variable
  'n.distinct.UMIs'
annotateOffTargets: no visible binding for global variable
  'offTarget_Start'
getAlnWithBulge : <anonymous>: no visible binding for global variable
  'pa.f1'
getAlnWithBulge : <anonymous>: no visible binding for global variable
  'pa.r2'
getPeaks: no visible binding for global variable 'adjusted.p.value'
getPeaks: no visible binding for global variable 'SNratio'
getUniqueCleavageEvents: no visible binding for global variable
  'width.first'
getUniqueCleavageEvents: no visible binding for global variable
  'width.last'
getUniqueCleavageEvents: no visible binding for global variable
  'qwidth.last'
getUniqueCleavageEvents: no visible binding for global variable
  'strand.last'
getUniqueCleavageEvents: no visible binding for global variable
  'qwidth.first'
getUniqueCleavageEvents: no visible binding for global variable
  'strand.first'
getUniqueCleavageEvents: no visible binding for global variable
  'readName'
getUniqueCleavageEvents: no visible binding for global variable
  'seqnames.last'
getUniqueCleavageEvents: no visible binding for global variable
  'seqnames.first'
getUniqueCleavageEvents: no visible binding for global variable
  'start.last'
getUniqueCleavageEvents: no visible binding for global variable
  'end.first'
getUniqueCleavageEvents: no visible binding for global variable 'UMI'
getUniqueCleavageEvents: no visible binding for global variable
  'end.last'
getUniqueCleavageEvents: no visible binding for global variable
  'start.first'
offTargetAnalysisOfPeakRegions: no visible binding for global variable
  'thePeak'
offTargetAnalysisOfPeakRegions: no visible binding for global variable
  'gRNAPlusPAM'
offTargetAnalysisOfPeakRegions: no visible binding for global variable
  'offTarget'
plotAlignedOfftargets: no visible binding for global variable
  'peak_score'
plotAlignedOfftargets: no visible binding for global variable 'MR'
plotAlignedOfftargets: no visible binding for global variable
  'guideAlignment2OffTarget'
plotAlignedOfftargets: no visible binding for global variable 'y'
plotAlignedOfftargets: no visible binding for global variable 'h'
plotHeatmapOfftargets: no visible binding for global variable
  'Offtargets'
plotHeatmapOfftargets: no visible binding for global variable 'MR'
plotHeatmapOfftargets: no visible binding for global variable 'MR.all'
plotHeatmapOfftargets: no visible binding for global variable 'Samples'
plotHeatmapOfftargets: no visible global function definition for 'unit'
plotTracks: no visible binding for global variable 'offTarget_Start'
plotTracks: no visible binding for global variable 'offTarget_End'
Undefined global functions or variables:
  .getMatchedInd DNA_BASES IUPAC_CODE_MAP MR MR.all Offtargets SNratio
  Samples UMI adjusted.p.value chromosome end.first end.last feature
  gRNA.deletion gRNA.insertion gRNA.name gRNAPlusPAM
  guideAlignment2OffTarget h mismatch.distance2PAM n.DNA.bulge
  n.RNA.bulge n.deletion n.distinct.UMIs n.guide.mismatch n.insertion
  offTarget offTargetStrand offTarget_End offTarget_Start
  offTarget_sequence pa.f1 pa.r2 peak_score pos.deletion pos.insertion
  predicted_cleavage_score qwidth.first qwidth.last readName
  seqnames.first seqnames.last start.first start.last strand.first
  strand.last thePeak unit width.first width.last y
* checking Rd files ... NOTE
prepare_Rd: annotateOffTargets.Rd:35-37: Dropping empty section \details
prepare_Rd: annotateOffTargets.Rd:63-65: Dropping empty section \references
prepare_Rd: createBarcodeFasta.Rd:34-36: Dropping empty section \value
prepare_Rd: createBarcodeFasta.Rd:59-61: Dropping empty section \references
prepare_Rd: getUsedBarcodes.Rd:53-55: Dropping empty section \references
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of ‘data’ directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking sizes of PDF files under ‘inst/doc’ ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
                     user system elapsed
PEtagAnalysis      13.669  0.733  14.481
annotateOffTargets  5.924  0.257   6.201
* checking for unstated dependencies in ‘tests’ ... OK
* checking tests ...
  Running ‘testthat.R’
 OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE

Status: 4 NOTEs
See
  ‘/Users/biocbuild/bbs-3.16-bioc/meat/GUIDEseq.Rcheck/00check.log’
for details.



Installation output

GUIDEseq.Rcheck/00install.out

##############################################################################
##############################################################################
###
### Running command:
###
###   /Library/Frameworks/R.framework/Resources/bin/R CMD INSTALL GUIDEseq
###
##############################################################################
##############################################################################


* installing to library ‘/Library/Frameworks/R.framework/Versions/4.2/Resources/library’
* installing *source* package ‘GUIDEseq’ ...
** using staged installation
** R
** data
** inst
** byte-compile and prepare package for lazy loading
** help
*** installing help indices
** building package indices
** installing vignettes
** testing if installed package can be loaded from temporary location
** testing if installed package can be loaded from final location
** testing if installed package keeps a record of temporary installation path
* DONE (GUIDEseq)

Tests output

GUIDEseq.Rcheck/tests/testthat.Rout


R version 4.2.3 (2023-03-15) -- "Shortstop Beagle"
Copyright (C) 2023 The R Foundation for Statistical Computing
Platform: x86_64-apple-darwin17.0 (64-bit)

R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.

R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.

Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.

> library(testthat)
> library(GUIDEseq)
Loading required package: GenomicRanges
Loading required package: stats4
Loading required package: BiocGenerics

Attaching package: 'BiocGenerics'

The following objects are masked from 'package:stats':

    IQR, mad, sd, var, xtabs

The following objects are masked from 'package:base':

    Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
    as.data.frame, basename, cbind, colnames, dirname, do.call,
    duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
    lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
    pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
    tapply, union, unique, unsplit, which.max, which.min

Loading required package: S4Vectors

Attaching package: 'S4Vectors'

The following objects are masked from 'package:base':

    I, expand.grid, unname

Loading required package: IRanges
Loading required package: GenomeInfoDb
> library(org.Hs.eg.db)
Loading required package: AnnotationDbi
Loading required package: Biobase
Welcome to Bioconductor

    Vignettes contain introductory material; view with
    'browseVignettes()'. To cite Bioconductor, see
    'citation("Biobase")', and for packages 'citation("pkgname")'.


> 
> test_check("GUIDEseq")
Loading required package: GenomicFeatures
Loading required package: BSgenome
Loading required package: Biostrings
Loading required package: XVector

Attaching package: 'Biostrings'

The following object is masked from 'package:base':

    strsplit

Loading required package: rtracklayer
GUIDEseqAnalysis without bulge. 
Remove duplicate reads ...

Peak calling ...

computing coverage for plus strand ...
computing coverage for minus strand ...
call peaks ...
finding local max for chromosome: chr13
combine plus and minus peaks ... 

keep peaks not in merged.gr but present in both peaks1 and peaks2

Find unmerged peaks with very high reads one-library protocol

offtarget analysis ...

search for gRNAs for input file1...
[1] "Scoring ..."
>>> Finding all hits in sequence chr13+:27629413:27629420:chr13-:27629400:27629404 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:39262927:39262939:chr13-:39262918:39262920 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:30964712:30964730 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:67161908:67161923 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:29087136:29087137 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:115084360:115084375 ...
>>> DONE searching
finish off-target search in sequence 2
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
Extract PAM sequence and n.PAM.mismatch. 
Done with offtarget search!
Add gene and exon information to offTargets ....
Order offtargets. 
Add sequence depth information. 

Get the number of unique UMIs for each offtarget. 

Save offtargets. 
Please check output file in directory PEtagTestResults

GUIDEseqAnalysis with offtargets containing no bulge and with bulge. 
Remove duplicate reads ...

Peak calling ...

computing coverage for plus strand ...
computing coverage for minus strand ...
call peaks ...
finding local max for chromosome: chr13
combine plus and minus peaks ... 

keep peaks not in merged.gr but present in both peaks1 and peaks2

Find unmerged peaks with very high reads one-library protocol

offtarget analysis ...

search for gRNAs for input file1...
[1] "Scoring ..."
>>> Finding all hits in sequence chr13+:27629413:27629420:chr13-:27629400:27629404 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:39262927:39262939:chr13-:39262918:39262920 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:30964712:30964730 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:67161908:67161923 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:29087136:29087137 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:115084360:115084375 ...
>>> DONE searching
finish off-target search in sequence 2
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
Extract PAM sequence and n.PAM.mismatch. 
Finding offtargets with bulges ...Done with offtarget search!
Add gene and exon information to offTargets ....
Order offtargets. 
Add sequence depth information. 

Get the number of unique UMIs for each offtarget. 

Save offtargets. 
Please check output file in directory PEtagTestResults

GUIDEseqAnalysis with bulge containing offtargets where no offtargets contain no bulge. 
Remove duplicate reads ...

Peak calling ...

computing coverage for plus strand ...
computing coverage for minus strand ...
call peaks ...
finding local max for chromosome: chr13
combine plus and minus peaks ... 

keep peaks not in merged.gr but present in both peaks1 and peaks2

Find unmerged peaks with very high reads one-library protocol

offtarget analysis ...

search for gRNAs for input file1...
[1] "Scoring ..."
>>> Finding all hits in sequence chr13+:27629413:27629420:chr13-:27629400:27629404 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:39262927:39262939:chr13-:39262918:39262920 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:30964712:30964730 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:66687218:66687225 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:67161908:67161923 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:98226906:98226924 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:29087136:29087137 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:97395652:97395666 ...
>>> DONE searching
>>> Finding all hits in sequence chr13-:115084360:115084375 ...
>>> DONE searching
finish off-target search in sequence 2
finish off-target search in sequence 1
Remove duplicate reads ...

Peak calling ...

computing coverage for plus strand ...
computing coverage for minus strand ...
call peaks ...
finding local max for chromosome: chr13
combine plus and minus peaks ... 

keep peaks not in merged.gr but present in both peaks1 and peaks2

Find unmerged peaks with very high reads one-library protocol

offtarget analysis ...

search for gRNAs for input file1...
[1] "Scoring ..."
>>> Finding all hits in sequence chr13+:27629413:27629420:chr13-:27629399:27629417 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:39262920:39262939:chr13-:39262918:39262918 ...
>>> DONE searching
finish off-target search in sequence 2
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
Extract PAM sequence and n.PAM.mismatch. 
Done with offtarget search!
Add gene and exon information to offTargets ....
Order offtargets. 
Add sequence depth information. 

Get the number of unique UMIs for each offtarget. 

Save offtargets. 
Please check output file in directory PEtagTestResults

search for gRNAs for input file1...
[1] "Scoring ..."
>>> Finding all hits in sequence chr13+:27629413:27629420:chr13-:27629400:27629404 ...
>>> DONE searching
>>> Finding all hits in sequence chr13+:39262927:39262939:chr13-:39262918:39262920 ...
>>> DONE searching
finish off-target search in sequence 2
finish off-target search in sequence 1
finish feature vector building
finish score calculation
[1] "Done!"
bulge on gRNA and offtarget on the minus strand  worksStart testing offtarget on the minus strand ...test sequence not long enough for allowing large stretch of bulge[ FAIL 0 | WARN 7 | SKIP 0 | PASS 230 ]

[ FAIL 0 | WARN 7 | SKIP 0 | PASS 230 ]
> 
> proc.time()
   user  system elapsed 
 89.948   3.080  93.521 

Example timings

GUIDEseq.Rcheck/GUIDEseq-Ex.timings

nameusersystemelapsed
GUIDEseq-package0.0010.0010.002
GUIDEseqAnalysis0.0010.0010.001
PEtagAnalysis13.669 0.73314.481
annotateOffTargets5.9240.2576.201
buildFeatureVectorForScoringBulge0.0010.0000.000
combineOfftargets0.1000.0230.127
createBarcodeFasta0.0180.0020.020
getPeaks000
getUniqueCleavageEvents0.0010.0000.001
getUsedBarcodes0.0340.0020.036
mergePlusMinusPeaks0.0010.0000.000
offTargetAnalysisOfPeakRegions0.0010.0010.001
offTargetAnalysisWithBulge0.0000.0000.001
peaks.gr0.0260.0090.035
plotAlignedOfftargets0.2790.0110.292
plotHeatmapOfftargets0.0030.0010.003
plotTracks0.0010.0000.001
uniqueCleavageEvents0.0380.0090.047