DOI: 10.18129/B9.bioc.NxtIRFcore  

This package is for version 3.16 of Bioconductor; for the stable, up-to-date release version, see NxtIRFcore.

Core Engine for NxtIRF: a User-Friendly Intron Retention and Alternative Splicing Analysis using the IRFinder Engine

Bioconductor version: 3.16

Interactively analyses Intron Retention and Alternative Splicing Events (ASE) in RNA-seq data. NxtIRF quantifies ASE events in BAM files aligned to the genome using a splice-aware aligner such as STAR. The core quantitation algorithm relies on the IRFinder/C++ engine ported via Rcpp for multi-platform compatibility. In addition, NxtIRF provides convenient pipelines for downstream analysis and publication-ready visualisation tools. Note that NxtIRFcore is now replaced by SpliceWiz in Bioconductor 3.16 onwards.

Author: Alex Chit Hei Wong [aut, cre, cph], William Ritchie [cph], Ulf Schmitz [ctb]

Maintainer: Alex Chit Hei Wong <a.wong at centenary.org.au>

Citation (from within R, enter citation("NxtIRFcore")):


To install this package, start R (version "4.2") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:



HTML R Script NxtIRFcore: Differential Alternative Splicing and Intron Retention analysis
PDF   Reference Manual
Text   NEWS


biocViews AlternativeSplicing, Coverage, DifferentialSplicing, RNASeq, Software, Transcriptomics
Version 1.4.0
In Bioconductor since BioC 3.14 (R-4.1) (1.5 years)
License MIT + file LICENSE
Depends R (>= 3.5.0), NxtIRFdata
Imports methods, stats, utils, tools, parallel, magrittr, Rcpp (>= 1.0.5), data.table, fst, ggplot2, AnnotationHub, BiocFileCache, BiocGenerics, BiocParallel, Biostrings, BSgenome, DelayedArray, DelayedMatrixStats, genefilter, GenomeInfoDb, GenomicRanges, HDF5Array, IRanges, plotly, R.utils, rhdf5, rtracklayer, SummarizedExperiment, S4Vectors
LinkingTo Rcpp, zlibbioc, RcppProgress
Suggests knitr, rmarkdown, pheatmap, shiny, openssl, crayon, egg, DESeq2, limma, DoubleExpSeq, Rsubread, testthat (>= 3.0.0)
SystemRequirements C++11
URL https://github.com/alexchwong/NxtIRFcore
BugReports https://support.bioconductor.org/
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package NxtIRFcore_1.4.0.tar.gz
Windows Binary NxtIRFcore_1.4.0.zip (64-bit only)
macOS Binary (x86_64) NxtIRFcore_1.4.0.tgz
macOS Binary (arm64) NxtIRFcore_1.4.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/NxtIRFcore
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/NxtIRFcore
Bioc Package Browser https://code.bioconductor.org/browse/NxtIRFcore/
Package Short Url https://bioconductor.org/packages/NxtIRFcore/
Package Downloads Report Download Stats

Documentation »


R / CRAN packages and documentation

Support »

Please read the posting guide. Post questions about Bioconductor to one of the following locations: