Alex Chit Hei Wong
| Snapshot Date: 2024-06-26 14:00 -0400 (Wed, 26 Jun 2024) |
| git_url: https://git.bioconductor.org/packages/SpliceWiz |
| git_branch: RELEASE_3_19 |
| git_last_commit: 0677691 |
| git_last_commit_date: 2024-04-30 11:43:22 -0400 (Tue, 30 Apr 2024) |
| Back to Multiple platform build/check report for BioC 3.19: simplified long |
|
This page was generated on 2024-06-28 17:42 -0400 (Fri, 28 Jun 2024).
| Hostname | OS | Arch (*) | R version | Installed pkgs |
|---|---|---|---|---|
| nebbiolo1 | Linux (Ubuntu 22.04.3 LTS) | x86_64 | 4.4.0 (2024-04-24) -- "Puppy Cup" | 4760 |
| palomino3 | Windows Server 2022 Datacenter | x64 | 4.4.0 (2024-04-24 ucrt) -- "Puppy Cup" | 4494 |
| merida1 | macOS 12.7.4 Monterey | x86_64 | 4.4.0 (2024-04-24) -- "Puppy Cup" | 4508 |
| kjohnson1 | macOS 13.6.6 Ventura | arm64 | 4.4.0 (2024-04-24) -- "Puppy Cup" | 4466 |
| palomino7 | Windows Server 2022 Datacenter | x64 | 4.4.1 (2024-06-14 ucrt) -- "Race for Your Life" | 4362 |
| Click on any hostname to see more info about the system (e.g. compilers) (*) as reported by 'uname -p', except on Windows and Mac OS X | ||||
| Package 2054/2300 | Hostname | OS / Arch | INSTALL | BUILD | CHECK | BUILD BIN | ||||||||
| SpliceWiz 1.6.0 (landing page) Alex Chit Hei Wong
| nebbiolo1 | Linux (Ubuntu 22.04.3 LTS) / x86_64 | OK | ERROR | skipped | |||||||||
| palomino3 | Windows Server 2022 Datacenter / x64 | OK | OK | OK | OK | | ||||||||
| merida1 | macOS 12.7.4 Monterey / x86_64 | OK | ERROR | skipped | skipped | |||||||||
| kjohnson1 | macOS 13.6.6 Ventura / arm64 | OK | OK | OK | OK | | ||||||||
| palomino7 | Windows Server 2022 Datacenter / x64 | OK | ERROR | skipped | skipped | |||||||||
|
To the developers/maintainers of the SpliceWiz package: - Allow up to 24 hours (and sometimes 48 hours) for your latest push to git@git.bioconductor.org:packages/SpliceWiz.git to reflect on this report. See Troubleshooting Build Report for more information. - Use the following Renviron settings to reproduce errors and warnings. - If 'R CMD check' started to fail recently on the Linux builder(s) over a missing dependency, add the missing dependency to 'Suggests:' in your DESCRIPTION file. See Renviron.bioc for more information. |
| Package: SpliceWiz |
| Version: 1.6.0 |
| Command: F:\biocbuild\bbs-3.19-bioc\R\bin\R.exe CMD check --no-multiarch --install=check:SpliceWiz.install-out.txt --library=F:\biocbuild\bbs-3.19-bioc\R\library --no-vignettes --timings SpliceWiz_1.6.0.tar.gz |
| StartedAt: 2024-06-27 06:31:45 -0400 (Thu, 27 Jun 2024) |
| EndedAt: 2024-06-27 06:48:04 -0400 (Thu, 27 Jun 2024) |
| EllapsedTime: 979.6 seconds |
| RetCode: 0 |
| Status: OK |
| CheckDir: SpliceWiz.Rcheck |
| Warnings: 0 |
##############################################################################
##############################################################################
###
### Running command:
###
### F:\biocbuild\bbs-3.19-bioc\R\bin\R.exe CMD check --no-multiarch --install=check:SpliceWiz.install-out.txt --library=F:\biocbuild\bbs-3.19-bioc\R\library --no-vignettes --timings SpliceWiz_1.6.0.tar.gz
###
##############################################################################
##############################################################################
* using log directory 'F:/biocbuild/bbs-3.19-bioc/meat/SpliceWiz.Rcheck'
* using R version 4.4.0 (2024-04-24 ucrt)
* using platform: x86_64-w64-mingw32
* R was compiled by
gcc.exe (GCC) 13.2.0
GNU Fortran (GCC) 13.2.0
* running under: Windows Server 2022 x64 (build 20348)
* using session charset: UTF-8
* using option '--no-vignettes'
* checking for file 'SpliceWiz/DESCRIPTION' ... OK
* this is package 'SpliceWiz' version '1.6.0'
* package encoding: UTF-8
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking whether package 'SpliceWiz' can be installed ... OK
* used C++ compiler: 'G__~1.EXE (GCC) 13.2.0'
* checking C++ specification ... NOTE
Specified C++11: please drop specification unless essential
* checking installed package size ... OK
* checking package directory ... OK
* checking 'build' directory ... OK
* checking DESCRIPTION meta-information ... NOTE
License stub is invalid DCF.
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking code files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking whether startup messages can be suppressed ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking line endings in shell scripts ... OK
* checking line endings in C/C++/Fortran sources/headers ... OK
* checking line endings in Makefiles ... OK
* checking compilation flags in Makevars ... OK
* checking for GNU extensions in Makefiles ... OK
* checking for portable use of $(BLAS_LIBS) and $(LAPACK_LIBS) ... OK
* checking use of PKG_*FLAGS in Makefiles ... OK
* checking compiled code ... NOTE
Note: information on .o files for x64 is not available
File 'F:/biocbuild/bbs-3.19-bioc/R/library/SpliceWiz/libs/x64/SpliceWiz.dll':
Found '_assert', possibly from 'assert' (C)
Found '_exit', possibly from '_exit' (C)
Found 'abort', possibly from 'abort' (C), 'runtime' (Fortran)
Found 'exit', possibly from 'exit' (C), 'stop' (Fortran)
Compiled code should not call entry points which might terminate R nor
write to stdout/stderr instead of to the console, nor use Fortran I/O
nor system RNGs nor [v]sprintf. The detected symbols are linked into
the code but might come from libraries and not actually be called.
See 'Writing portable packages' in the 'Writing R Extensions' manual.
* checking files in 'vignettes' ... OK
* checking examples ... OK
Examples with CPU (user + system) or elapsed time > 5s
user system elapsed
Build-Reference-methods 59.56 2.44 71.44
NxtSE-class 55.25 2.14 61.12
ASE-methods 31.00 0.31 31.33
covPlotObject-class 25.39 0.65 26.02
plotCoverage 16.34 0.44 16.78
Run_SpliceWiz_Filters 8.10 0.01 8.16
* checking for unstated dependencies in 'tests' ... OK
* checking tests ...
Running 'testthat.R'
OK
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes ... OK
* checking running R code from vignettes ... SKIPPED
* checking re-building of vignette outputs ... SKIPPED
* checking PDF version of manual ... OK
* DONE
Status: 3 NOTEs
See
'F:/biocbuild/bbs-3.19-bioc/meat/SpliceWiz.Rcheck/00check.log'
for details.
SpliceWiz.Rcheck/00install.out
############################################################################## ############################################################################## ### ### Running command: ### ### F:\biocbuild\bbs-3.19-bioc\R\bin\R.exe CMD INSTALL SpliceWiz ### ############################################################################## ############################################################################## * installing to library 'F:/biocbuild/bbs-3.19-bioc/R/library' * installing *source* package 'SpliceWiz' ... ** using staged installation ** libs using C++ compiler: 'G__~1.EXE (GCC) 13.2.0' using C++11 g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c BAM2blocks.cpp -o BAM2blocks.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c FastaReader.cpp -o FastaReader.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c FragmentBlocks.cpp -o FragmentBlocks.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c GZTools.cpp -o GZTools.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c RcppExports.cpp -o RcppExports.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c ReadBlockProcessor.cpp -o ReadBlockProcessor.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c ReadBlockProcessor_CoverageBlocks.cpp -o ReadBlockProcessor_CoverageBlocks.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c ReadBlockProcessor_FragmentsMap.cpp -o ReadBlockProcessor_FragmentsMap.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c ReadBlockProcessor_TandemJunctions.cpp -o ReadBlockProcessor_TandemJunctions.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c covTools.cpp -o covTools.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c main.cpp -o main.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c swEngine.cpp -o swEngine.o g++ -std=gnu++11 -I"F:/biocbuild/bbs-3.19-bioc/R/include" -DNDEBUG -I'F:/biocbuild/bbs-3.19-bioc/R/library/ompBAM/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/Rcpp/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/include' -I'F:/biocbuild/bbs-3.19-bioc/R/library/RcppProgress/include' -I"C:/rtools44/x86_64-w64-mingw32.static.posix/include" -fopenmp -DSPLICEWIZ -O2 -Wall -mfpmath=sse -msse2 -mstackrealign -c synthReadGenerator.cpp -o synthReadGenerator.o g++ -shared -s -static-libgcc -o SpliceWiz.dll tmp.def BAM2blocks.o FastaReader.o FragmentBlocks.o GZTools.o RcppExports.o ReadBlockProcessor.o ReadBlockProcessor_CoverageBlocks.o ReadBlockProcessor_FragmentsMap.o ReadBlockProcessor_TandemJunctions.o covTools.o main.o swEngine.o synthReadGenerator.o -fopenmp -DSPLICEWIZ -LF:/biocbuild/bbs-3.19-bioc/R/library/zlibbioc/libs/x64 -lzlib1bioc -LC:/rtools44/x86_64-w64-mingw32.static.posix/lib/x64 -LC:/rtools44/x86_64-w64-mingw32.static.posix/lib -LF:/biocbuild/bbs-3.19-bioc/R/bin/x64 -lR installing to F:/biocbuild/bbs-3.19-bioc/R/library/00LOCK-SpliceWiz/00new/SpliceWiz/libs/x64 ** R ** inst ** byte-compile and prepare package for lazy loading ** help *** installing help indices ** building package indices ** installing vignettes ** testing if installed package can be loaded from temporary location ** testing if installed package can be loaded from final location ** testing if installed package keeps a record of temporary installation path * DONE (SpliceWiz)
SpliceWiz.Rcheck/tests/testthat.Rout
R version 4.4.0 (2024-04-24 ucrt) -- "Puppy Cup"
Copyright (C) 2024 The R Foundation for Statistical Computing
Platform: x86_64-w64-mingw32/x64
R is free software and comes with ABSOLUTELY NO WARRANTY.
You are welcome to redistribute it under certain conditions.
Type 'license()' or 'licence()' for distribution details.
R is a collaborative project with many contributors.
Type 'contributors()' for more information and
'citation()' on how to cite R or R packages in publications.
Type 'demo()' for some demos, 'help()' for on-line help, or
'help.start()' for an HTML browser interface to help.
Type 'q()' to quit R.
> library(testthat)
> library(SpliceWiz)
Loading required package: NxtIRFdata
SpliceWiz package loaded with 2 threads
Use setSWthreads() to set the number of SpliceWiz threads
>
> test_check("SpliceWiz")
Loading required package: Rsubread
Jun 27 6:43:09 AM Converting FASTA to local TwoBitFile...done
Jun 27 6:43:10 AM Connecting to genome TwoBitFile...done
Jun 27 6:43:10 AM Making local copy of GTF file...done
Jun 27 6:43:11 AM Extracting temp genome FASTA from TwoBit file
Jun 27 6:43:12 AM Generating synthetic reads, saving to F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\refWithMapExcl/Mappability/Reads.fa
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
10145 synthetic reads generated
Jun 27 6:43:12 AM Cleaning temp genome / gene annotation files
========== _____ _ _ ____ _____ ______ _____
===== / ____| | | | _ \| __ \| ____| /\ | __ \
===== | (___ | | | | |_) | |__) | |__ / \ | | | |
==== \___ \| | | | _ <| _ /| __| / /\ \ | | | |
==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/
Rsubread 2.18.0
//================================= setting ==================================\\
|| ||
|| Index name : reference_index ||
|| Index space : base space ||
|| Index split : no-split ||
|| Repeat threshold : 100 repeats ||
|| Gapped index : no ||
|| ||
|| Free / total memory : 38.9GB / 64.0GB ||
|| ||
|| Input files : 1 file in total ||
|| o genome.fa ||
|| ||
\\============================================================================//
//================================= Running ==================================\\
|| ||
|| Check the integrity of provided reference sequences ... ||
|| No format issues were found ||
|| Scan uninformative subreads in reference sequences ... ||
|| Estimate the index size... ||
|| 8%, 0 mins elapsed, rate=28.9k bps/s ||
|| 16%, 0 mins elapsed, rate=57.0k bps/s ||
|| 24%, 0 mins elapsed, rate=84.6k bps/s ||
|| 33%, 0 mins elapsed, rate=111.2k bps/s ||
|| 41%, 0 mins elapsed, rate=137.6k bps/s ||
|| 49%, 0 mins elapsed, rate=163.4k bps/s ||
|| 58%, 0 mins elapsed, rate=188.4k bps/s ||
|| 66%, 0 mins elapsed, rate=212.8k bps/s ||
|| 74%, 0 mins elapsed, rate=236.7k bps/s ||
|| 83%, 0 mins elapsed, rate=260.3k bps/s ||
|| 91%, 0 mins elapsed, rate=283.6k bps/s ||
|| 3.0 GB of memory is needed for index building. ||
|| Build the index... ||
|| 8%, 0 mins elapsed, rate=3.6k bps/s ||
|| 16%, 0 mins elapsed, rate=7.1k bps/s ||
|| 24%, 0 mins elapsed, rate=10.7k bps/s ||
|| 33%, 0 mins elapsed, rate=14.2k bps/s ||
|| 41%, 0 mins elapsed, rate=17.7k bps/s ||
|| 49%, 0 mins elapsed, rate=21.2k bps/s ||
|| 58%, 0 mins elapsed, rate=24.7k bps/s ||
|| 66%, 0 mins elapsed, rate=28.1k bps/s ||
|| 74%, 0 mins elapsed, rate=31.6k bps/s ||
|| 83%, 0 mins elapsed, rate=35.0k bps/s ||
|| 91%, 0 mins elapsed, rate=38.4k bps/s ||
|| Save current index block... ||
|| [ 0.0% finished ] ||
|| [ 10.0% finished ] ||
|| [ 20.0% finished ] ||
|| [ 30.0% finished ] ||
|| [ 40.0% finished ] ||
|| [ 50.0% finished ] ||
|| [ 60.0% finished ] ||
|| [ 70.0% finished ] ||
|| [ 80.0% finished ] ||
|| [ 90.0% finished ] ||
|| [ 100.0% finished ] ||
|| ||
|| Total running time: 0.3 minutes. ||
||Index F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\refWithMapExcl\refe ... ||
|| ||
\\============================================================================//
========== _____ _ _ ____ _____ ______ _____
===== / ____| | | | _ \| __ \| ____| /\ | __ \
===== | (___ | | | | |_) | |__) | |__ / \ | | | |
==== \___ \| | | | _ <| _ /| __| / /\ \ | | | |
==== ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
========== |_____/ \____/|____/|_| \_\______/_/ \_\_____/
Rsubread 2.18.0
//================================= setting ==================================\\
|| ||
|| Function : Read alignment + Junction detection (RNA-Seq) ||
|| Input file : Reads.fa ||
|| Output file : AlignedReads.bam (BAM) ||
|| Index name : reference_index ||
|| ||
|| ------------------------------------ ||
|| ||
|| Threads : 1 ||
|| Phred offset : 33 ||
|| Min votes : 1 / 14 ||
|| Max mismatches : 3 ||
|| Max indel length : 5 ||
|| Report multi-mapping reads : yes ||
|| Max alignments per multi-mapping read : 1 ||
|| Annotations : transcripts.gtf (GTF) ||
|| ||
\\============================================================================//
//================= Running (27-Jun-2024 06:43:29, pid=2780) =================\\
|| ||
|| Check the input reads. ||
|| The input file contains base space reads. ||
|| Initialise the memory objects. ||
|| Estimate the mean read length. ||
|| Create the output BAM file. ||
|| Check the index. ||
|| Init the voting space. ||
|| Load the annotation file. ||
|| 538 annotation records were loaded. ||
|| Global environment is initialised. ||
|| Load the 1-th index block... ||
|| The index block has been loaded. ||
|| Start read mapping in chunk. ||
|| 65% completed, 0.6 mins elapsed, rate=25.2k reads per second ||
|| 83% completed, 0.6 mins elapsed, rate=0.2k reads per second ||
|| 99% completed, 0.6 mins elapsed, rate=0.3k reads per second ||
|| ||
|| Completed successfully. ||
|| ||
\\==================================== ====================================//
//================================ Summary =================================\\
|| ||
|| Total reads : 10145 ||
|| Mapped : 10145 (100.0%) ||
|| Uniquely mapped : 10145 ||
|| Multi-mapping : 0 ||
|| ||
|| Unmapped : 0 ||
|| ||
|| Junctions : 0 ||
|| Indels : 1 ||
|| ||
|| Running time : 0.6 minutes ||
|| ||
\\============================================================================//
Jun 27 6:44:04 AM Calculating Mappability Exclusion regions from: F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq/refWithMapExcl/Mappability/AlignedReads.bam
Calculating Mappability Exclusions: F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\refWithMapExcl\Mappability\AlignedReads.bam
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing Mappability Exclusions
**************************************************|
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Jun 27 6:44:09 AM Reference generated without non-polyA reference
Jun 27 6:44:09 AM Reference generated without Mappability reference
Jun 27 6:44:09 AM Reference generated without Blacklist exclusion
Jun 27 6:44:09 AM Converting FASTA to local TwoBitFile...done
Jun 27 6:44:10 AM Connecting to genome TwoBitFile...done
Jun 27 6:44:10 AM Making local copy of GTF file...done
Jun 27 6:44:11 AM Reading source GTF file...done
Jun 27 6:44:12 AM Processing gtf file...
...genes
...transcripts
...CDS
...exons
done
Jun 27 6:44:13 AM Gene ontology not prepared for this reference
Jun 27 6:44:15 AM Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Jun 27 6:44:22 AM Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Jun 27 6:44:31 AM Predicting NMD transcripts from genome sequence
...exonic transcripts
...retained introns
|
| | 0%
|
|======================================================================| 100%
done
Jun 27 6:44:34 AM Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Jun 27 6:44:36 AM Splice Annotations Filtered
Jun 27 6:44:37 AM Translating Alternate Splice Peptides...done
Jun 27 6:44:37 AM Splice Annotations finished
Reference build finished
Jun 27 6:44:38 AM Running SpliceWiz processBAM
Reading reference file
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
Processing BAM file F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Performing final sort of fragment maps
Writing COV file
**************************************************|
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam processed (510 milliseconds)
Jun 27 6:44:39 AM Running SpliceWiz processBAM
Reading reference file
Allocating memory to 2 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
Processing BAM file F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Compiling data from threads
Performing final sort of fragment maps
Writing COV file
**************************************************|
0% 10 20 30 40 50 60 70 80 90 100%
[----|----|----|----|----|----|----|----|----|----|
**************************************************|
Writing output file
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam processed (325 milliseconds)
Jun 27 6:44:39 AM Reference generated without non-polyA reference
Jun 27 6:44:39 AM Reference generated without Mappability reference
Jun 27 6:44:39 AM Reference generated without Blacklist exclusion
Jun 27 6:44:39 AM Converting FASTA to local TwoBitFile...done
Jun 27 6:44:41 AM Connecting to genome TwoBitFile...done
Jun 27 6:44:41 AM Making local copy of GTF file...done
Jun 27 6:44:41 AM Reading source GTF file...done
Jun 27 6:44:42 AM Processing gtf file...
...genes
...transcripts
...CDS
Jun 27 6:44:42 AM No protein information detected in reference! For full functionality, ensure there are valid entries with type == `CDS` in the gtf file. Protein reference and NMD annotation is skipped.
...exons
done
Jun 27 6:44:43 AM Gene ontology not prepared for this reference
Jun 27 6:44:45 AM Processing introns...
...data
...basic annotations
...splice motifs
...other info
...defining flanking exon clusters
done
Jun 27 6:44:49 AM Generating processBAM reference
...prepping data
...determining measurable introns (directional)
...determining measurable introns (non-directional)
...writing ref-cover.bed
...writing ref-ROI.bed
...writing ref-read-continues.ref
...writing ref-sj.ref
...writing ref-tj.ref
processBAM reference generated
Jun 27 6:44:57 AM Annotating Splice Events
Annotating Mutually-Exclusive-Exon Splice Events...done
Annotating Skipped-Exon Splice Events...done
Annotating Alternate 5' / 3' Splice Site Splice Events...done
Annotating Alternate First / Last Exon Splice Events...done
Annotating known retained introns...done
Jun 27 6:44:59 AM Splice Annotations Filtered
Reference build finished
Jun 27 6:45:01 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam processed (457 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H025.bam processed (382 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H026.bam processed (662 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H033.bam processed (632 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H043.bam processed (375 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H046.bam processed (478 milliseconds)
Jun 27 6:45:04 AM Validating Experiment; checking COV files...
Jun 27 6:45:04 AM Compiling Sample Stats
Jun 27 6:45:04 AM Compiling Junction List...merging...done
Jun 27 6:45:05 AM Compiling Junction Stats...merging...done
Jun 27 6:45:14 AM Compiling Intron Retention List...done
Jun 27 6:45:15 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...copying splicing reference
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done
Jun 27 6:45:27 AM Generating NxtSE assays
Jun 27 6:45:44 AM Building Final NxtSE Object
Jun 27 6:45:44 AM ...consolidating assays to H5 file
Jun 27 6:45:46 AM ...packaging reference
Jun 27 6:45:46 AM ...synthesising NxtSE
Jun 27 6:45:46 AM ...determining how overlapping introns should be removed
Jun 27 6:45:50 AM SpliceWiz (NxtSE) Collation Finished
Jun 27 6:45:50 AM Loading NxtSE object from file...
Jun 27 6:45:50 AM ...loading reference (NxtSE)
Jun 27 6:45:50 AM ...linking assays
Jun 27 6:45:50 AM ...linking COV files
Jun 27 6:45:50 AM ...loading rowData
Jun 27 6:45:50 AM ...removing overlapping introns...
Jun 27 6:45:50 AM NxtSE loaded
Jun 27 6:45:51 AM SpliceWiz reference already exists in given directory
Jun 27 6:45:51 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (0 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam processed (521 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H025.bam processed (436 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H026.bam processed (564 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H033.bam processed (563 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H043.bam processed (384 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H046.bam processed (589 milliseconds)
Jun 27 6:45:54 AM Validating Experiment; checking COV files...
Jun 27 6:45:54 AM Compiling Sample Stats
Jun 27 6:45:54 AM Compiling Junction List...merging...done
Jun 27 6:45:56 AM Compiling Junction Stats...merging...done
Jun 27 6:46:04 AM Compiling Intron Retention List...done
Jun 27 6:46:05 AM Compiling Tandem Junction List...merging...done
Jun 27 6:46:06 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...looking for novel exons
Jun 27 6:46:10 AM Assembling novel splicing reference:
...loading reference FASTA/GTF
...injecting novel transcripts to GTF
...processing GTF
...processing introns from GTF
...annotating alternative splicing events
done
Jun 27 6:46:23 AM Tidying up splice junctions and intron retentions (part 2)...
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done
Jun 27 6:46:33 AM Generating NxtSE assays
Jun 27 6:46:50 AM Building Final NxtSE Object
Jun 27 6:46:50 AM ...consolidating assays to H5 file
Jun 27 6:46:52 AM ...packaging reference
Jun 27 6:46:52 AM ...synthesising NxtSE
Jun 27 6:46:53 AM ...determining how overlapping introns should be removed
Jun 27 6:46:56 AM SpliceWiz (NxtSE) Collation Finished
Jun 27 6:46:56 AM Loading NxtSE object from file...
Jun 27 6:46:56 AM ...loading reference (NxtSE)
Jun 27 6:46:56 AM ...linking assays
Jun 27 6:46:56 AM ...linking COV files
Jun 27 6:46:56 AM ...loading rowData
Jun 27 6:46:56 AM ...removing overlapping introns...
Jun 27 6:46:57 AM NxtSE loaded
Jun 27 6:46:57 AM SpliceWiz reference already exists in given directory
Jun 27 6:46:57 AM Running SpliceWiz processBAM
Allocating memory to 1 threads for SpliceWiz (ompBAM)...initialized (1 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H003.bam processed (535 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H025.bam processed (432 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H026.bam processed (650 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H033.bam processed (623 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H043.bam processed (407 milliseconds)
F:\biocbuild\bbs-3.19-bioc\tmpdir\Rtmp23pxDq\02H046.bam processed (579 milliseconds)
Jun 27 6:47:00 AM Validating Experiment; checking COV files...
Jun 27 6:47:00 AM Compiling Sample Stats
Jun 27 6:47:00 AM Compiling Junction List...merging...done
Jun 27 6:47:02 AM Compiling Junction Stats...merging...done
Jun 27 6:47:10 AM Compiling Intron Retention List...done
Jun 27 6:47:11 AM Tidying up splice junctions and intron retentions...
...annotating splice junctions
...copying splicing reference
...grouping splice junctions
...grouping introns
...loading splice events
...compiling rowEvents
done
Jun 27 6:47:23 AM Generating NxtSE assays
Jun 27 6:47:39 AM Building Final NxtSE Object
Jun 27 6:47:39 AM ...consolidating assays to H5 file
Jun 27 6:47:42 AM ...packaging reference
Jun 27 6:47:42 AM ...synthesising NxtSE
Jun 27 6:47:42 AM ...determining how overlapping introns should be removed
Jun 27 6:47:45 AM SpliceWiz (NxtSE) Collation Finished
Jun 27 6:47:45 AM Loading NxtSE object from file...
Jun 27 6:47:45 AM ...loading reference (NxtSE)
Jun 27 6:47:45 AM ...linking assays
Jun 27 6:47:46 AM ...linking COV files
Jun 27 6:47:46 AM ...loading rowData
Jun 27 6:47:46 AM ...removing overlapping introns...
Jun 27 6:47:46 AM NxtSE loaded
path paired strand depth mean_frag_size
02H003 ../SpliceWiz_Output/02H003.txt.gz TRUE -1 106256 149.3108
02H025 ../SpliceWiz_Output/02H025.txt.gz TRUE -1 84872 151.5914
02H026 ../SpliceWiz_Output/02H026.txt.gz TRUE -1 131514 150.3238
02H033 ../SpliceWiz_Output/02H033.txt.gz TRUE -1 129013 153.9754
02H043 ../SpliceWiz_Output/02H043.txt.gz TRUE -1 80254 155.4004
02H046 ../SpliceWiz_Output/02H046.txt.gz TRUE -1 120174 151.2840
directionality_strength Intergenic_Fraction rRNA_Fraction
02H003 9850 0 0
02H025 9848 0 0
02H026 9848 0 0
02H033 9855 0 0
02H043 9841 0 0
02H046 9857 0 0
NonPolyA_Fraction Mitochondrial_Fraction Unanno_Jn_Fraction
02H003 0 0 0.013449472
02H025 0 0 0.011203515
02H026 0 0 0.007282382
02H033 0 0 0.006310241
02H043 0 0 0.005871040
02H046 0 0 0.009819533
NMD_Jn_Fraction Fraction_Splice_Reads Fraction_Span_Reads IRBurden_clean
02H003 0.019509970 0.3506908 0.1735243 0.05796583
02H025 0.019360162 0.3712414 0.1591691 0.03963531
02H026 0.004298323 0.3980261 0.1615037 0.02576549
02H033 0.007705562 0.4003550 0.1734089 0.03090263
02H043 0.028069104 0.3671219 0.1807511 0.03762138
02H046 0.010542303 0.3725598 0.1603508 0.05099352
IRBurden_exitrons IRBurden_clean_unstranded IRBurden_exitrons_unstranded
02H003 0.1291810 0.03844293 0.1296020
02H025 0.1192351 0.02730399 0.1194005
02H026 0.1231055 0.02010410 0.1231939
02H033 0.1378958 0.02300500 0.1380215
02H043 0.1392935 0.02286515 0.1397365
02H046 0.1132136 0.03359077 0.1133852
IRBurden_antisense
02H003 0.021393335
02H025 0.013191532
02H026 0.006007119
02H033 0.008453742
02H043 0.015837096
02H046 0.019043703
[ FAIL 0 | WARN 0 | SKIP 0 | PASS 45 ]
>
> # bump to trigger r cmd check
>
> proc.time()
user system elapsed
275.45 14.23 299.18
SpliceWiz.Rcheck/SpliceWiz-Ex.timings
| name | user | system | elapsed | |
| ASE-GLM-edgeR | 2.92 | 0.12 | 3.05 | |
| ASE-methods | 31.00 | 0.31 | 31.33 | |
| ASEFilter-class | 0.06 | 0.00 | 0.06 | |
| Build-Reference-methods | 59.56 | 2.44 | 71.44 | |
| Coverage | 1.21 | 0.06 | 1.33 | |
| Gene-ontology-methods | 2.42 | 0.12 | 2.53 | |
| Graphics-User-Interface | 0 | 0 | 0 | |
| Mappability-methods | 2.73 | 0.04 | 3.56 | |
| NxtSE-class | 55.25 | 2.14 | 61.12 | |
| Run_SpliceWiz_Filters | 8.10 | 0.01 | 8.16 | |
| STAR-methods | 0 | 0 | 0 | |
| View-Reference-methods | 0.23 | 0.00 | 0.34 | |
| collateData | 0.39 | 0.14 | 0.54 | |
| coord2GR | 0.03 | 0.02 | 0.04 | |
| covDataObject-class | 2.10 | 0.00 | 2.22 | |
| covPlotObject-class | 25.39 | 0.65 | 26.02 | |
| covPlotly-class | 1.12 | 0.07 | 1.18 | |
| example-SpliceWiz-data | 0.00 | 0.01 | 0.02 | |
| findSamples | 0.00 | 0.02 | 0.02 | |
| isCOV | 0.02 | 0.01 | 0.03 | |
| makeSE | 0.62 | 0.30 | 0.92 | |
| make_plot_data | 0.05 | 0.00 | 0.05 | |
| plotCoverage | 16.34 | 0.44 | 16.78 | |
| processBAM | 2.86 | 0.23 | 1.92 | |
| setSWthreads | 0 | 0 | 0 | |
| theme_white | 0.19 | 0.00 | 0.19 | |