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Assessment of evidence for LOH in spatial transcriptomics pre-processed data using Bayes factor calculations

Bioconductor version: Release (3.19)

tLOH, or transcriptomicsLOH, assesses evidence for loss of heterozygosity (LOH) in pre-processed spatial transcriptomics data. This tool requires spatial transcriptomics cluster and allele count information at likely heterozygous single-nucleotide polymorphism (SNP) positions in VCF format. Bayes factors are calculated at each SNP to determine likelihood of potential loss of heterozygosity event. Two plotting functions are included to visualize allele fraction and aggregated Bayes factor per chromosome. Data generated with the 10X Genomics Visium Spatial Gene Expression platform must be pre-processed to obtain an individual sample VCF with columns for each cluster. Required fields are allele depth (AD) with counts for reference/alternative alleles and read depth (DP).

Author: Michelle Webb [cre, aut], David Craig [aut]

Maintainer: Michelle Webb <michelgw at>

Citation (from within R, enter citation("tLOH")):


To install this package, start R (version "4.4") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:

tLOH HTML R Script
Reference Manual PDF


biocViews CopyNumberVariation, GeneExpression, SNP, Software, Transcription, Transcriptomics
Version 1.12.0
In Bioconductor since BioC 3.13 (R-4.1) (3 years)
License MIT + file LICENSE
Depends R (>= 4.2)
Imports scales, stats, utils, ggplot2, data.table, purrr, dplyr, VariantAnnotation, GenomicRanges, MatrixGenerics, bestNormalize, depmixS4, naniar, stringr
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Source Package tLOH_1.12.0.tar.gz
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