smokingMouse 1.5.1
Welcome to the smokingMouse
project.
In this vignette we’ll show you how to access the smoking-nicotine-mouse LIBD datasets.
You can find the analysis code and the data generation in here.
The main motivation to create this Bioconductor package was to provide public and free access to all RNA-seq datasets that were generated for the smoking-nicotine-mouse project, containing variables of interest that make it possible to answer a wide range of biological questions related to smoking and nicotine effects in mice.
This bulk RNA-sequencing project consisted of a differential expression analysis (DEA) involving 4 data types: genes, transcripts, exons, and exon-exon junctions. The main goal of this study was to explore the effects of prenatal exposure to smoking and nicotine on the developing mouse brain. As secondary objectives, this work evaluated: 1) the affected genes by each exposure in the adult female brain in order to compare offspring and adult results, and 2) the effects of smoking on adult blood and brain to search for overlapping biomarkers in both tissues. Finally, DEGs identified in mice were compared against previously published results in human (Semick et al., 2020 and Toikumo et al., 2023).