MaxContrastProjection

DOI: 10.18129/B9.bioc.MaxContrastProjection    

This package is for version 3.8 of Bioconductor; for the stable, up-to-date release version, see MaxContrastProjection.

Perform a maximum contrast projection of 3D images along the z-dimension into 2D

Bioconductor version: 3.8

A problem when recording 3D fluorescent microscopy images is how to properly present these results in 2D. Maximum intensity projections are a popular method to determine the focal plane of each pixel in the image. The problem with this approach, however, is that out-of-focus elements will still be visible, making edges and fine structures difficult to detect. This package aims to resolve this problem by using the contrast around a given pixel to determine the focal plane, allowing for a much cleaner structure detection than would be otherwise possible. For convenience, this package also contains functions to perform various other types of projections, including a maximum intensity projection.

Author: Jan Sauer, Bernd Fischer

Maintainer: Jan Sauer <jan.sauer at dkfz-heidelberg.de>

Citation (from within R, enter citation("MaxContrastProjection")):

Installation

To install this package, start R (version "3.5") and enter:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install("MaxContrastProjection")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("MaxContrastProjection")

 

PDF R Script MaxContrastProjection
PDF   Reference Manual
Text   NEWS

Details

biocViews CellBasedAssays, ImmunoOncology, Preprocessing, Software, Visualization
Version 1.6.1
In Bioconductor since BioC 3.5 (R-3.4) (2 years)
License Artistic-2.0
Depends R (>= 3.4)
Imports EBImage, stats, methods
LinkingTo
Suggests knitr, BiocStyle, testthat
SystemRequirements GNU make
Enhances
URL
Depends On Me
Imports Me
Suggests Me
Links To Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package MaxContrastProjection_1.6.1.tar.gz
Windows Binary MaxContrastProjection_1.6.1.zip
Mac OS X 10.11 (El Capitan) MaxContrastProjection_1.6.1.tgz
Source Repository git clone https://git.bioconductor.org/packages/MaxContrastProjection
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/MaxContrastProjection
Package Short Url http://bioconductor.org/packages/MaxContrastProjection/
Package Downloads Report Download Stats

Documentation »

Bioconductor

R / CRAN packages and documentation

Support »

Please read the posting guide. Post questions about Bioconductor to one of the following locations: