DOI: 10.18129/B9.bioc.isobar  

This package is for version 3.16 of Bioconductor; for the stable, up-to-date release version, see isobar.

Analysis and quantitation of isobarically tagged MSMS proteomics data

Bioconductor version: 3.16

isobar provides methods for preprocessing, normalization, and report generation for the analysis of quantitative mass spectrometry proteomics data labeled with isobaric tags, such as iTRAQ and TMT. Features modules for integrating and validating PTM-centric datasets (isobar-PTM). More information on

Author: Florian P Breitwieser < at> and Jacques Colinge <jacques.colinge at>, with contributions from Alexey Stukalov <stukalov at>, Xavier Robin <xavier.robin at> and Florent Gluck <florent.gluck at>

Maintainer: Florian P Breitwieser < at>

Citation (from within R, enter citation("isobar")):


To install this package, start R (version "4.2") and enter:

if (!require("BiocManager", quietly = TRUE))


For older versions of R, please refer to the appropriate Bioconductor release.


To view documentation for the version of this package installed in your system, start R and enter:



PDF R Script isobar for developers
PDF R Script isobar for quantification of PTM datasets
PDF R Script isobar package for iTRAQ and TMT protein quantification
PDF R Script Usecases for isobar package
PDF   Reference Manual
Text   NEWS


biocViews Bioinformatics, ImmunoOncology, MassSpectrometry, MultipleComparisons, Proteomics, QualityControl, Software
Version 1.44.0
In Bioconductor since BioC 2.9 (R-2.14) (11.5 years)
License LGPL-2
Depends R (>= 2.10.0), Biobase, stats, methods
Imports distr, plyr, biomaRt, ggplot2
Suggests MSnbase, OrgMassSpecR, XML, RJSONIO, Hmisc, gplots, RColorBrewer, gridExtra, limma, boot, DBI, MASS
Depends On Me
Imports Me
Suggests Me RforProteomics
Links To Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package isobar_1.44.0.tar.gz
Windows Binary
macOS Binary (x86_64) isobar_1.44.0.tgz
macOS Binary (arm64) isobar_1.44.0.tgz
Source Repository git clone
Source Repository (Developer Access) git clone
Bioc Package Browser
Package Short Url
Package Downloads Report Download Stats

Documentation »


R / CRAN packages and documentation

Support »

Please read the posting guide. Post questions about Bioconductor to one of the following locations: