CRISPRseek

DOI: 10.18129/B9.bioc.CRISPRseek    

Design of target-specific guide RNAs in CRISPR-Cas9, genome-editing systems

Bioconductor version: Release (3.11)

The package includes functions to find potential guide RNAs for the CRISPR editing system including Base Editors and the Prime Editor for input target sequences, optionally filter guide RNAs without restriction enzyme cut site, or without paired guide RNAs, genome-wide search for off-targets, score, rank, fetch flank sequence and indicate whether the target and off-targets are located in exon region or not. Potential guide RNAs are annotated with total score of the top5 and topN off-targets, detailed topN mismatch sites, restriction enzyme cut sites, and paired guide RNAs. This package leverages Biostrings and BSgenome packages.

Author: Lihua Julie Zhu, Benjamin R. Holmes, Hervé Pagès, Michael Lawrence, Isana Veksler-Lublinsky, Victor Ambros, Neil Aronin and Michael Brodsky

Maintainer: Lihua Julie Zhu <julie.zhu at umassmed.edu>

Citation (from within R, enter citation("CRISPRseek")):

Installation

To install this package, start R (version "4.0") and enter:

if (!requireNamespace("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("CRISPRseek")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("CRISPRseek")

 

PDF R Script CRISPRseek Vignette
PDF   Reference Manual
Text   NEWS

Details

biocViews CRISPR, GeneRegulation, ImmunoOncology, SequenceMatching, Software
Version 1.28.0
In Bioconductor since BioC 2.14 (R-3.1) (6.5 years)
License GPL (>= 2)
Depends R (>= 3.0.1), BiocGenerics, Biostrings
Imports parallel, data.table, seqinr, S4Vectors(>= 0.9.25), IRanges, BSgenome, BiocParallel, hash, methods
LinkingTo
Suggests RUnit, BiocStyle, BSgenome.Hsapiens.UCSC.hg19, TxDb.Hsapiens.UCSC.hg19.knownGene, org.Hs.eg.db
SystemRequirements
Enhances
URL
Depends On Me crisprseekplus
Imports Me GUIDEseq
Suggests Me
Links To Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package CRISPRseek_1.28.0.tar.gz
Windows Binary CRISPRseek_1.28.0.zip
macOS 10.13 (High Sierra) CRISPRseek_1.28.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/CRISPRseek
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/CRISPRseek
Package Short Url https://bioconductor.org/packages/CRISPRseek/
Package Downloads Report Download Stats
Old Source Packages for BioC 3.11 Source Archive

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