############################################################################## ############################################################################## ### ### Running command: ### ### /home/biocbuild/bbs-3.19-bioc/R/bin/R CMD build --keep-empty-dirs --no-resave-data breakpointR ### ############################################################################## ############################################################################## * checking for file ‘breakpointR/DESCRIPTION’ ... OK * preparing ‘breakpointR’: * checking DESCRIPTION meta-information ... OK * installing the package to build vignettes * creating vignettes ... ERROR --- re-building ‘breakpointR.Rnw’ using knitr breakpointr package:breakpointR R Documentation _M_a_i_n _f_u_n_c_t_i_o_n _f_o_r _t_h_e '_b_r_e_a_k_p_o_i_n_t_R' _p_a_c_k_a_g_e _D_e_s_c_r_i_p_t_i_o_n: This function is an easy-to-use wrapper to find breakpoints with 'runBreakpointr' in parallel, write the results to file, plot results and find hotspots. _U_s_a_g_e: breakpointr( inputfolder, outputfolder, configfile = NULL, numCPU = 1, reuse.existing.files = FALSE, windowsize = 1e+06, binMethod = "size", multi.sizes = NULL, pairedEndReads = FALSE, pair2frgm = FALSE, chromosomes = NULL, min.mapq = 10, filtAlt = FALSE, genoT = "fisher", trim = 10, peakTh = 0.33, zlim = 3.291, background = 0.05, minReads = 10, maskRegions = NULL, callHotSpots = FALSE, conf = 0.99 ) _A_r_g_u_m_e_n_t_s: inputfolder: Folder with BAM files. outputfolder: Folder to output the results. If it does not exist it will be created. configfile: A file specifying the parameters of this function (without 'inputfolder', 'outputfolder' and 'configfile'). Having the parameters in a file can be handy if many samples with the same parameter settings are to be run. If a 'configfile' is specified, it will take priority over the command line parameters. numCPU: The numbers of CPUs that are used. Should not be more than available on your machine. reuse.existing.files: A logical indicating whether or not existing files in 'outputfolder' should be reused. windowsize: The window size used to calculate deltaWs, either number of reads or genomic size depending on 'binMethod'. binMethod: Method used to calculate optimal number of reads in the window ("size", "reads"). By default 'binMethod='size''. multi.sizes: User defined multiplications of the original window size. pairedEndReads: Set to 'TRUE' if you have paired-end reads in your file. pair2frgm: Set to 'TRUE' if every paired-end read should be merged into a single fragment. chromosomes: If only a subset of the chromosomes should be binned, specify them here. min.mapq: Minimum mapping quality when importing from BAM files. filtAlt: Set to 'TRUE' if you want to filter out alternative alignments defined in 'XA' tag. genoT: A method ('fisher' or 'binom') to genotype regions defined by a set of breakpoints. trim: The amount of outliers in deltaWs removed to calculate the stdev (10 will remove top 10% and bottom 10% of deltaWs). peakTh: The treshold that the peak deltaWs must pass to be considered a breakpoint (e.g. 0.33 is 1/3 of max(deltaW)). zlim: The number of stdev that the deltaW must pass the peakTh (ensures only significantly higher peaks are considered). background: The percent (e.g. 0.05 = 5%) of background reads allowed for WW or CC genotype calls. minReads: The minimal number of reads between two breaks required for genotyping. maskRegions: List of regions to be excluded from the analysis (tab-separated file: chromosomes start end). callHotSpots: Search for regions of high abundance of breakpoints in single cells. conf: Desired confidence interval of localized breakpoints. _V_a_l_u_e: 'NULL' _A_u_t_h_o_r(_s): David Porubsky, Aaron Taudt, Ashley Sanders _E_x_a_m_p_l_e_s: ## Not run: ## The following call produces plots and genome browser files for all BAM files in "my-data-folder" breakpointr(inputfolder="my-data-folder", outputfolder="my-output-folder") ## End(Not run) Error: processing vignette 'breakpointR.Rnw' failed with diagnostics: Running 'texi2dvi' on 'breakpointR.tex' failed. LaTeX errors: ! Package xcolor Error: Undefined color `fgcolor'. See the xcolor package documentation for explanation. Type H for immediate help. ... ! Emergency stop. ... l.58 ...}{rgb}{0.941, 0.941, 0.941}\color{fgcolor} \begin{kframe} ! ==> Fatal error occurred, no output PDF file produced! --- failed re-building ‘breakpointR.Rnw’ SUMMARY: processing the following file failed: ‘breakpointR.Rnw’ Error: Vignette re-building failed. Execution halted