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### Running command:
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###   /home/biocbuild/bbs-3.16-bioc/R/bin/R CMD check --install=check:LowMACA.install-out.txt --library=/home/biocbuild/bbs-3.16-bioc/R/site-library --timings LowMACA_1.28.0.tar.gz
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* using log directory ‘/home/biocbuild/bbs-3.16-bioc/meat/LowMACA.Rcheck’
* using R version 4.2.3 (2023-03-15)
* using platform: x86_64-pc-linux-gnu (64-bit)
* using session charset: UTF-8
* checking for file ‘LowMACA/DESCRIPTION’ ... OK
* checking extension type ... Package
* this is package ‘LowMACA’ version ‘1.28.0’
* checking package namespace information ... OK
* checking package dependencies ... OK
* checking if this is a source package ... OK
* checking if there is a namespace ... OK
* checking for hidden files and directories ... OK
* checking for portable file names ... OK
* checking for sufficient/correct file permissions ... OK
* checking whether package ‘LowMACA’ can be installed ... OK
* checking installed package size ... OK
* checking package directory ... OK
* checking ‘build’ directory ... OK
* checking DESCRIPTION meta-information ... OK
* checking top-level files ... OK
* checking for left-over files ... OK
* checking index information ... OK
* checking package subdirectories ... OK
* checking R files for non-ASCII characters ... OK
* checking R files for syntax errors ... OK
* checking whether the package can be loaded ... OK
* checking whether the package can be loaded with stated dependencies ... OK
* checking whether the package can be unloaded cleanly ... OK
* checking whether the namespace can be loaded with stated dependencies ... OK
* checking whether the namespace can be unloaded cleanly ... OK
* checking loading without being on the library search path ... OK
* checking dependencies in R code ... OK
* checking S3 generic/method consistency ... OK
* checking replacement functions ... OK
* checking foreign function calls ... OK
* checking R code for possible problems ... OK
* checking Rd files ... OK
* checking Rd metadata ... OK
* checking Rd cross-references ... OK
* checking for missing documentation entries ... OK
* checking for code/documentation mismatches ... OK
* checking Rd \usage sections ... OK
* checking Rd contents ... OK
* checking for unstated dependencies in examples ... OK
* checking contents of ‘data’ directory ... OK
* checking data for non-ASCII characters ... OK
* checking data for ASCII and uncompressed saves ... OK
* checking files in ‘vignettes’ ... OK
* checking examples ... ERROR
Running examples in ‘LowMACA-Ex.R’ failed
The error most likely occurred in:

> base::assign(".ptime", proc.time(), pos = "CheckExEnv")
> ### Name: alignSequences
> ### Title: Align sequences via clustalo
> ### Aliases: alignSequences
> 
> ### ** Examples
> 
> #Create an object of class LowMACA for RAS domain family
> lm <- newLowMACA(pfam="PF00071" , genes=c("KRAS" , "NRAS" , "HRAS"))
All Gene Symbols correct!
> #Align sequences using local installation of clustalo
> lm <- alignSequences(lm)
Aligning sequences...
> #Web service clustalomega aligner
> lm <- alignSequences(lm , mail="lowmaca@gmail.com")
Aligning sequences...
Checking perl installation...
Checking perl modules XML::Simple and LWP...
JobId: clustalo-R20230411-024435-0305-79733356-p2m
QUEUED
http status: 400 Bad Request  Job 'clustalo-R20230411-024435-0305-79733356-p2m' is still queued at /home/biocbuild/bbs-3.16-bioc/R/site-library/LowMACA/clustalo_lwp.pl line 262.
Warning in system(exec, intern = TRUE) :
  running command ''/usr/bin/perl' '/home/biocbuild/bbs-3.16-bioc/R/site-library/LowMACA/clustalo_lwp.pl' --noguidetreeout --stype protein --dismatout --outfmt clustal --email lowmaca@gmail.com --outfile '/tmp/RtmpT7QTW4/webClustal' '/tmp/RtmpT7QTW4/file37961247a386dd'' had status 255
Error in .clustalOAlign(genesData, clustal_cmd, clustalo_filename, mail,  : 
  Alignment with ClustalOmega had non 0 exit status:
Calls: alignSequences -> alignSequences -> .clustalOAlign
Execution halted
* checking for unstated dependencies in vignettes ... OK
* checking package vignettes in ‘inst/doc’ ... OK
* checking running R code from vignettes ...
  ‘LowMACA.Rmd’ using ‘UTF-8’... OK
 NONE
* checking re-building of vignette outputs ... OK
* checking PDF version of manual ... OK
* DONE

Status: 1 ERROR
See
  ‘/home/biocbuild/bbs-3.16-bioc/meat/LowMACA.Rcheck/00check.log’
for details.