############################################################################## ############################################################################## ### ### Running command: ### ### chmod a+r piano -R && D:\biocbuild\bbs-3.15-bioc\R\bin\R.exe CMD build --keep-empty-dirs --no-resave-data piano ### ############################################################################## ############################################################################## * checking for file 'piano/DESCRIPTION' ... OK * preparing 'piano': * checking DESCRIPTION meta-information ... OK * installing the package to build vignettes * creating vignettes ... ERROR --- re-building 'Running_gene-set_analysis_with_piano.Rmd' using rmarkdown Running gene set analysis: Final gene/gene-set association: 1136 genes and 50 gene sets Details: Calculating gene set statistics from 1136 out of 2000 gene-level statistics Using all 2000 gene-level statistics for significance estimation Removed 0 genes from GSC due to lack of matching gene statistics Removed 0 gene sets containing no genes after gene removal Removed additionally 0 gene sets not matching the size limits Loaded additional information for 0 gene sets --- finished re-building 'Running_gene-set_analysis_with_piano.Rmd' --- re-building 'piano-vignette.Rnw' using Sweave Loading data in text file... ...done Loading setup file... ...done Text file data: No normalization performed. Creating annotation... ...done Removing unmapped probes... ..done Warning in runQC(myArrayData) : can not run rnaDeg: argument arrayData does not contain dataRaw Warning in runQC(myArrayData) : can not run nuseRle: argument arrayData does not contain dataRaw Generating normalized data distribution plot... ...done Generating normalized data boxplot... dev.new(): using pdf(file="Rplots1.pdf") ...done Generating PCA... ...done dev.new(): using pdf(file="Rplots2.pdf") dev.new(): using pdf(file="Rplots3.pdf") dev.new(): using pdf(file="Rplots4.pdf") dev.new(): using pdf(file="Rplots5.pdf") Generating PCA... ...done dev.new(): using pdf(file="Rplots6.pdf") dev.new(): using pdf(file="Rplots7.pdf") dev.new(): using pdf(file="Rplots8.pdf") dev.new(): using pdf(file="Rplots9.pdf") Generating PCA... ...done dev.new(): using pdf(file="Rplots10.pdf") dev.new(): using pdf(file="Rplots11.pdf") dev.new(): using pdf(file="Rplots12.pdf") dev.new(): using pdf(file="Rplots13.pdf") Fitting linear models... ...done Generating Venn diagrams... dev.new(): using pdf(file="Rplots14.pdf") dev.new(): using pdf(file="Rplots15.pdf") ...done Calculating p-values for contrast aerobic_Clim - anaerobic_Clim... Error: processing vignette 'piano-vignette.Rnw' failed with diagnostics: chunk 10 Error in `[.data.frame`(topTab, , c("ID", "tmp", "logFC", "AveExpr", "t", : undefined columns selected --- failed re-building 'piano-vignette.Rnw' SUMMARY: processing the following file failed: 'piano-vignette.Rnw' Error: Vignette re-building failed. Execution halted